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For cell stimulation, cells were pretreated with various concentrations of GHK-Cu of 1, 5, or 10 µM for 18 hours, followed by stimulation with 100 ng/ml LPS for specific time periods

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Surprisingly, a substantial amount of flavokavains A and B (4.35%) was reported in this kava formula, which appeared to be different from the other reports about aqueous kava preparations [5]